Abstract
Measurement of cytosolic calcium concentration in protoplasts of bean leaf was carried out using Fluo-3/AM indicator. The uptake of the fluorescent indicator, as its permeable ester, was achieved by employing pluronic acid F-127 in the incubating solution. In the absence of pluronic acid F-127 loading was two times lower in the same time period. A rapid intracellular location of the fluorescent dye after intracellular hydrolysis is reported, with no vacuole trapping of the fluorescent indicator. An increase from 54 to 91 nM free calcium ([Ca2+]i) was observed immediately after addition of a pectic elicitor. Advantages of the use of pluronic acid F-127 are in the speed of the indicator entry, eliminating any possible hydrolysis by external esterases, and more efficient loading while maintaining cell integrity. © 1997 John Wiley & Sons, Ltd.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
