Use of plant growth-promoting rhizobacteria isolates as a potential biofertiliser for wheat

Abstract

Plant growth-promoting rhizobacteria (PGPR) isolated from the rhizosphere soil of eight field crops at different locations in Egypt were identified. Rhizobacteria strains were identified as Bacillus endophyticus AW1 5, B. filamentosus EM9, ET3, Micrococcus luteus KT2, FW9, FC13, SaW4, Enterobacter cloacae SK18, Pseudomonas azotoformans TPo10, Citrobacter braakii TC3. All isolates solubilised insoluble phosphate and produced IAA, while only six were able to produce siderophores in vitro. Vegetative growth and yield of wheat cv. ‘Sakha 94’ were enhanced after the application of single inoculation of each isolate compared to the control. Grain yield was increased by 20.7– 96.5% over the control according to bacterial isolates. Available phosphorus (P) and counts of total bacteria in soil were observed to be significantly increased in treatments than in control. After the wheat harvest, soil pH was observed to be decreased, and a highly significant negative correlation was observed between soil pH and the levels of available phosphorus. Significant increases in grain and straw yields, as well as uptake of nitrogen (N) and P by plants, were observed due to inoculation with PGPR isolates. Levels of photosynthetic pigments, free amino acids, free phenolics, and reducing sugars in flag leaf and spikes were significantly enhanced by the application of all PGPR isolates compared to the control. Thus this study identifies the PGPR isolates for the improvement of the growth, yield, and quality of wheat. The study may be also useful for field evaluation under different soils and environmental conditions before generalising PGPR isolates as biofertilisers.