Abstract
The objective of this study was to determine the use of phytone peptone to optimize the growth and cell density of Lactobacillus reuteri. Four strains of L. reuteri (DSM 20016, SD 2112, CF 2-7F, and MF 2-3,) were used in this study. An overnight culture of individual strains was inoculated into fresh basal media with various protein sources (peptone, tryptone, proteose peptone #3, phytone peptone, tryptic soy broth, yeast extract, and beef extract). Samples were then mixed well and incubated at 37 °C for 15 h. Bacterial growth was monitored by measuring turbidity (optical density 610 nm) at different time intervals during the incubation period. At the end of incubation, samples were plated on de-Man Rogosa Sharpe (MRS) agar to determine the bacterial population. Our results showed that phytone peptone promoted the growth of L. reuteri (p < 0.05) by 1.4 log CFU/mL on average compared to the control samples. Therefore, phytone peptone could be included in laboratory media to enhance growth and increase the cell density of L. reuteri.
Highlights
Lactobacillus reuteri is used as a probiotic to promote health and improve the body’s defenses against medical conditions and illnesses such as gastrointestinal tract problems, atopic disease, colic, and oral gingivitis [1,2,3,4]
The turbidity readings and bacterial count for L. reuteri DSM 20016 reached a maximum absorbance of 1.35, and the population increased by 1.65 log CFU/mL and reached 10.05 log CFU/mL (p < 0.05) when GF5-phytone peptone was added (Figure 1a, Table 2)
When basal media was supplemented with protein sources including GF1, GF2, GF4, and GF7, there was a slight but not significant (p > 0.05) growth compared to the control sample
Summary
Lactobacillus reuteri is used as a probiotic to promote health and improve the body’s defenses against medical conditions and illnesses such as gastrointestinal tract problems, atopic disease, colic, and oral gingivitis [1,2,3,4]. L. reuteri has a variety of beneficial effects on human health, it has had limited application in commercial dairy foods. High cell density cultivations have become increasingly important as several companies have developed an interest in lactic acid bacteria (LAB). It has been shown that high cell density is necessary in order to obtain the health benefits conferred by probiotics [6,7]. We have defined growth-promoting factors for probiotic strains as protein sources that promote the growth of bacterial cells that impact probiotic functionality [9,10]. The functionality of probiotics, including lactic acid bacteria, is determined by the presence of growth-promoting factors. Lactic acid bacteria strains predominantly use peptides to meet their demand for complex nitrogen [11]. Van Niel and Hahn-Hägerdal [12]
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