Abstract
Physcion is well known for the treatment of carcinoma. However, the therapeutic effect of physcion on atopic dermatitis (AD) through the inhibition of thymic stromal lymphopoietin (TSLP) level remains largely unknown. In this study, we investigated the anti-AD effect of physcion using HMC-1 cells, splenocytes, and a murine model. Treatment with physcion decreased production and mRNA expression levels of TSLP, IL-6, TNF-α, and IL-1β in activated HMC-1 cells. Physcion reduced the expression levels of RIP2/caspase-1 and phospho (p)ERK/pJNK/pp38 in activated HMC-1 cells. Physcion suppressed the expression levels of pIKKβ/NF-κB/pIkBα in activated HMC-1 cells. Moreover, physcion attenuated the production levels of TSLP, IL-4, IL-6, TNF-α, and IFN-γ from activated splenocytes. Oral administration of physcion improved the severity of 2,4-dinitrochlorobenzene-induced AD-like lesional skin through reducing infiltration of inflammatory cells and mast cells, and the protein and mRNA levels of TSLP, IL-4, and IL-6 in the lesional skin tissues. Physcion attenuated histamine, IgE, TSLP, IL-4, IL-6, and TNF-α levels in serum. In addition, physcion inhibited caspase-1 activation in the lesional skin tissues. These findings indicate that physcion could ameliorate AD-like skin lesions by inhibiting TSLP levels via caspase-1/MAPKs/NF-kB signalings, which would provide experimental evidence of the therapeutic potential of physcion for AD.
Highlights
Atopic dermatitis (AD) is one of the most common relapsing inflammatory skin diseases in the world [1]
Effects of physcion on thymic stromal lymphopoietin (TSLP) levels from HMC-1 cells as well as on atopic dermatitis (AD)-like skin lesions have not been in this study, we investigated whether physcion could downregulate the TSLP level in human mast determined
We found that physcion inhibits TSLP levels as well as inflammatory cytokines levels in activated mast cells
Summary
Atopic dermatitis (AD) is one of the most common relapsing inflammatory skin diseases in the world [1]. Infiltration and activation of mast cells were upregulated in various AD animal role in atopic disorders [8]. The caspase-1/nuclear factor (NF)-κB signal cascade controlled TSLP expression in HMC-1 cells [15]. Physcion has various anthraquinone derivative, is present in vegetables such as cabbage lettuce and beans [16] Physcion pharmacological properties, such as anti-inflammatory, anti-microbial, and anti-tumor [17]. Effects of physcion on TSLP levels from HMC-1 cells as well as on AD-like skin lesions have not been in this study, we investigated whether physcion could downregulate the TSLP level in human mast determined. In this study, we investigated whether physcion could downregulate the TSLP cell line, HMC-1 cells and mice. Level in human mast cell line, HMC-1 cells and mice
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