Use of periodically hCG hormones injection for the gonadal development of java barb (Puntius javanicus) as bioreproduction applied on aquaculture

Abstract

Tawes fish or Java barb (Puntius javanicus Bleeker, 1855) as the one of the endogenous species in Indonesia has specific reproductive roles that its cycle still depends on local environment. It makes limited frequency of spawning in nature that has been decreasing seeds production either domestication or cultivation. As a seasonal spawner, it needs long time for gonadal development. Gonadal development of endogenous species broodstock by hormone is needed to improve bio-reproductive capacity and it will cause faster gonadal maturity of Java Barb. One of exogenous hormones that could trigger gonadotropin hormone (GtH) is the human chorionic gonadotropin (hCG) hormone and it will accelerate the maturity level of the gonad by periodically injection. The aim of research is to determine the effect and the best dosage of hCG hormone for the gonadal development of Java barb. This study used an experimental method with a completely randomized design, with 4 treatments and 3 repetitions in 30 days observed. 48 broodstock with 200 gram average weight were used and induced by hCG. The doses of hCG are 0, 100, 200 and 300 IU/kg. The parameters observed include the level of gonad maturity, gonado somatic index (GSI) and hepatosomatic index (HSI). After statistical analysis, result showed that dose of 200 IU could improve the best development of gonad in Java barb base on 3rd maturity level achieved, gonado somatic index (10.11 ± 0.74%) and the hepatosomatic index at (1.99 ± 0.06%) respectively. The observation which used hCG hormone can improve gonadal development and one step closer to reproduce domesticated and cultived of Java Barb.