Abstract
Three methods utilizing PCR technology for the detection of members of the Geminiviridae are described. As well as being a tool useful for detection, the products of the diagnostic PCR reactions are suitable for further characterization of the viruses. Firstly, a pair of degenerate primers has been used to allow the detection of geminiviruses infecting dicotyledonous plants by PCR amplification. Secondly, a set of specific primers has been used which discriminate between the economically significant maize streak geminivirus and closely related viruses which infect mainly grasses. Finally, a PCR‐based method for the production of full‐length, infectious geminivirus clones is described. The advantages and disadvantages of PCR compared with other methods of detection and characterization are discussed.
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