Abstract
Japanese encephalitis (JE) and dengue are vector borne viral diseases that are endemic in the territory of Nepal. The purpose of the study was to assess the disease burden and to compare the results between particle agglutination and enzyme linked immunosorbent assay. A total of 185 serum samples of suspected Japanese encephalitis (JE) viral fever, dengue fever, malaria and typhoid were collected in the year 2005 (August-October) and 2006 (August- November) from hospitals of Bardiya, Banke, Dang, Kathmandu and Parsa. The samples were investigated by particle agglutination and enzyme immunoassay at Everest International Clinic and Research Centre, Kathmandu. Out of 141 samples from suspected diseased patients, 51% had a positive Japanese encephalitis virus (JEV) specific immunoglobulin M by particle agglutination assay while the anti-dengue immunoglobulin M positivity rate was 22.7% among 44 samples by the same assay. The specificity of particle agglutination kit against Japanese encephalitis and dengue was high as evident from absence of cross reactivity with other diseases like malaria, typhoid, kala-azar and leptospira. Thus, particle agglutination assay can be used as a tool for diagnosis of the diseases in developing countries like Nepal. Key words: dengue virus; endemic; Japanese encephalitis; particle agglutination DOI: 10.3126/njst.v11i0.4144Nepal Journal of Science and Technology 11 (2010) 189-192
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
