Use of oscillographic polarography in microbiology

Abstract

Oscillographic polarography with alternating current was used for following the course of utilisation and metabolism of some amino acids, purine and pyrimidine bases, similarly as their nucleosides and nucleotides in the washed suspensions of resting cells and in growing cultures ofEscherichia coli. The ‘Polaroscope P-524’ was used with mercury dropping electrode. The method was described for the oscillopolarographic investigation of activity of some bacterial enzymes, as for example: asparaginase, some nucleodesaminases and enzymes involved in the cleavage of nucleosides.