Use of nanoparticle form of milled teeth as a bone graft substitute in rabbits

Abstract

Aims of the study: This study was designed to determine the effects of milled tooth in its nanoparticle forms as a bone substitute materials on the acceleration of bone healing in rabbits. Materials and Methods: Sixteen albino rabbits were used in the experiment that were divided into two groups: nanoparticle group (M0 group) and control group. Rabbits were anesthetized using intramuscularly administered 10% ketamine (40mgkg) and 2% xylazine (5mgkg) (Inferchemi, Holland). After shaving and sterilization of the site of the operation, a 2cm submandibular incision was done. Then 3 millimeter circular anterior full-thickness defect was created near the midline of the mandible. The anterior defect was filled with 50-100nm milled tooth material nanoparticles. The incision was sutured with 3/0 black silk. Following surgery, each rabbit was given 30 mg/kg oxytetracycline intramuscularly. The rabbits were euthanized at 3, 7, 14, and 28 days afterwards. Then the specimens were dried and fixed in paraffin before being removed from the skulls. Histological examination the specimens was done.