Abstract
Three mutants of Colletotrichum lagenarium , with appressoria that could not penetrate nitrocellulose membranes or cell walls of the host plant, were obtained by mutagenic treatment. Appressoria of these mutants were identical in shape and colour to those of the parent strain. Scanning electron microscopy revealed that one mutant (83182) formed a penetration peg at the basal part of the appressoria and that the other mutants (82335 and 83348) did not form a penetration peg. The latter mutants are considered to have a defect in morphogenesis of the penetration peg. Application of Czapek liquid medium to mutant 83182 after appressorium formation partially restored appressorial penetration without halo formation; this mutant synthesized a protein, P-95, which was closely related to cellulose degrading enzymes. These results suggest that mutant 83182 is deficient in a secretion or translocation system for these enzymes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
