Use of Modified Lactobacillus Selective Medium and Bifidobacterium Iodoacetate Medium for Differential Enumeration of Lactobacillus acidophilus and Bifidobacterium spp. in Powdered Nutritional Products

Abstract

Modified Lactobacillus selective agar (APT agar + sodium acetate and glacial acetic acid; mLBS) was compared to selective modified Lactobacillus selective medium (LBS agar + tomato juice and acetic acid; mLSM) and nonselective de Man Rogosa Sharpe (MRS) agar for the enumeration of Lactobacillus acidophilus in probiotic-containing powdered nutritional products. The mLBS agar was equivalent to MRS agar and superior to the mLSM agar for enumerating L. acidophilus in products stored in sealed cans at 22°C. When samples were analyzed for L. acidophilus concentration after high temperature storage in sealed cans or storage in open cans at high relative humidity, the mLBS and MRS agars were highly correlated (r2 = 0.93). Modified Bifidobacterium iodoacetate medium (12.5 mg iodoacetic acid/liter; mBIM) was compared to MRS agar + bile, cysteine, and dicloxacillin (MRS + BCD) for enumerating Bifidobacterium infantis or Bifidobacterium lactis in the nutritional products. The two media were equivalent for enumerating B. infantis in product stored at 22°C in sealed cans. However, the two media were poorly correlated (r2 < 0.50) for enumeration of B. infantis and B. lactis in products stored in sealed cans at high temperature or in open cans at high relative humidity. The mLBS medium has potential industry application as a relatively inexpensive, convenient differential enumeration method for L. acidophilus. The mBIM medium cannot be recommended as a sole medium for enumeration of probiotic Bifidobacterium spp. in powdered nutritional products stored under high temperature and/or high relative humidity conditions.