Abstract
The use of microbore columns for high-speed chromatographic separation is investigated, and apparatus is described that can be usd with such columns. Specific details of detector cell construction are given, and photosensor specifications are suggested to provide the necessary fast detector response time. Specifications are also provided for appropriate data acquisition equipment. The performance of microbore columns operated at high mobile phase velocities is examined, and the effect of mobile phase velocity on column efficiency investigated. The precisions of quantitative analysis by peak height and peak area measurements are compared for a microbore columns operated at high linear mobile phase velocity. An example of the separation of a seven-component mixture in less than 30 sec is included.
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