Abstract
Imaging of increasingly complex cartilage in vertebrate embryos is one of the key tasks of developmental biology. This is especially important to study shape-organizing processes during initial skeletal formation and growth. Advanced imaging techniques that are reflecting biological needs give a powerful impulse to push the boundaries of biological visualization. Recently, techniques for contrasting tissues and organs have improved considerably, extending traditional 2D imaging approaches to 3D . X-ray micro computed tomography (μCT), which allows 3D imaging of biological objects including their internal structures with a resolution in the micrometer range, in combination with contrasting techniques seems to be the most suitable approach for non-destructive imaging of embryonic developing cartilage. Despite there are many software-based ways for visualization of 3D data sets, having a real solid model of the studied object might give novel opportunities to fully understand the shape-organizing processes in the developing body. In this feasibility study we demonstrated the full procedure of creating a real 3D object of mouse embryo nasal capsule, i.e. the staining, the μCT scanning combined by the advanced data processing and the 3D printing.
Highlights
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Despite there are many software-based ways for visualization of 3D data sets, having a real solid model of the studied object might give novel opportunities to fully understand the shape-organizing processes in the developing body. In this feasibility study we demonstrated the full procedure of creating a real 3D object of mouse embryo nasal capsule, i.e
The manual segmentation was realized in one direction across the sample, along the cross section shown in figures 1 and 5, denoted x, y in figure 5
Summary
This content has been downloaded from IOPscience. Please scroll down to see the full text. 2016 JINST 11 C03006 (http://iopscience.iop.org/1748-0221/11/03/C03006) View the table of contents for this issue, or go to the journal homepage for more. Despite there are many software-based ways for visualization of 3D data sets, having a real solid model of the studied object might give novel opportunities to fully understand the shape-organizing processes in the developing body The sequence of obtained projections is subsequently processed by tomographic reconstruction based on inverse Radon transform [8] In this way different X-ray absorption at each sample point is visualized and the 3D volume map of object density is created. Due to the cone shape of the X-ray beam, geometric magnification can be used to reach high-resolution [8]
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