Abstract
Mebendazole is an anthelmintic drug used in cattle production. However, residues may occur in produced food and in excretions, jeopardizing population health. A method based on micellar liquid chromatography (MLC) was developed to determine mebendazole in dairy products (milk, cheese, butter, and curd) and nitrogenous waste (urine and dung) from bovine animals. Sample treatment was expedited to simple dilution or solid-to-liquid extraction, followed by filtration and direct injection of the obtained solution. The analyte was resolved from matrix compounds in less than 8 min, using a C18 column and a mobile phase made up of 0.15 M sodium dodecyl sulfate (SDS)–6% 1-pentanol phosphate buffered at pH 7, and running at 1 mL/min under isocratic mode. Detection was performed by absorbance at 292 nm. The procedure was validated according to the guidelines of the EU Commission Decision 2002/657/EC in terms of: specificity, method calibration range (from the limit of quantification to 25–50 ppm), sensitivity (limit of detection 0.1–0.2 ppm; limit of quantification, 0.3–0.6 ppm), trueness (92.5–102.3%), precision (<7.5%, expressed at RSD), robustness, and stability. The method is reliable, sensitive, easy-to-handle, eco-friendly, safe, inexpensive, and provides a high sample-throughput. Therefore, it is useful for routine analysis as a screening or quantification method in a laboratory for drug-residue control.
Highlights
Livestock farming is one of the most sought after agri-businesses mainly due to changes in dietary pattern and ease in export policy of raw and prepared food products
The UV absorbance spectrum of MBZ was taken during the chromatographic run at the retention time, and the wavelength with the highest signal-to-noise ratio was found at the same wavelength
Trueness, precision, ruggedness, etc. were obtained, indicating the suitability of this method for the determination of MBZ in different edible products derived from bovine animal, which are regarded as complex biological matrices
Summary
Livestock farming is one of the most sought after agri-businesses mainly due to changes in dietary pattern and ease in export policy of raw and prepared food products. Poultry and cattle farming include the production of nutritious food such as egg, meat, milk, and other dairy products. In India, the rearing practices are done in traditional ways under natural conditions. In the current scenario, new breeds are raised to gain more productivity and profitability [1]. There are many challenges faced by the producer, especially issues related to cattle health and hygiene. Infectious diseases have a great impact on livestock and they directly affect
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