Abstract
Serious complications arise in the use of mercury-substituted nucleoside triphosphates to study chromatin transcription in vitro . In studying the transcription of duck reticulocyte chromatin by E. coli RNA polymerase, we find that endogenous globin RNA contaminates the “purified” de novo transcript, giving rise to false high levels of globin sequence abundance. The anomaly arises because the endogenous globin RNA acts as template for the polymerase, and forms a duplex with the Hg-substituted complementary strand that is made. Subsequent selection of Hg-containing RNA results in purification of the endogenous globin sequences.
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