Use of magnetic beads in Next-Generation Sequencing in the Construction of Low Cost 16S rRNA Gene Genomic Libraries

Abstract

This work aimed to test the new Mag-Bind® TotalPure magnetic beads in the construction of a genomic library of corn and sugarcane endophytes. The technique Next-Generation Sequencing (NGS) is very expensive and for that, one of the most expensive reagents are the magnetics beads that serve to purify the amplified DNA and separate by size depending on their proportion, as shown in the Illumina protocol. We observed that the use of these beads, which are five times cheaper and have the same capacity and quality as those on the market, are good for a satisfactory study of metagenomics and microbiomes. We conclude that the use of omega brand beads has an excellent result in the construction of a genomic library for NGS.