Abstract
Localized surface plasmon resonance (LSPR) spectroscopy has received extensive attention as a new method in chemical and biological analysis that can be integrated with nanotechnology. As a new analytical method, LSPR possesses various advantages over the traditional bioanalysis method of enzyme-linked immunosorbent assay (ELISA), including a label-free procedure, low cost, high response speed, simple operation and structure, and ease of storage and transport. Additionally, in situ and high-throughput measurements can be achieved. This study aims to solve the problem of the lack of in situ and highly efficient monitoring methods for current realgar bioleaching processes. An LSPR chip is made to monitor the changes in arsenic content in the process of realgar bioleaching. A convenient, fast, sensitive, and high-throughput method of bioleaching process investigation based on the LSPR spectroscopic in situ monitoring technique is proposed. The LSPR chip provided a highly specific selectivity and a linear detection of arsenic content in the range of 1.0–100.0 μM with detection limit (LOD) 0.898 μM. The developed chip was applied to the quantification of realgar bioleaching sample with satisfactory results.
Highlights
Bioleaching technology is a newly developed hydrometallurgical technology [1]. is technique is cross-fused with the preparation of medicinal realgar and uses natural acidophilic microorganisms to perform bioleaching [2]
Coating silver NPs with a layer of gold effectively protects the silver NPs from environmental influences and effectively improves the physical and chemical properties of the silver NPs [19]. erefore, this study provides a new solution to the difficulty of monitoring the change in arsenic content in the process of bioleaching realgar by producing an Localized surface plasmon resonance (LSPR) chip to investigate the bioleaching process via a convenient, rapid, sensitive, and highthroughput LSPR spectroscopic in situ monitoring technique
Realgar Bioleaching System. e strain A. ferrooxidans BY-3 (CCTCC-M203071) was obtained from the discarded copper mine in Baiyin City, Gansu Province, China, and purified by solid medium streak-plate separation. is strain was selected based on its growth and physical and chemical properties. e strain was entered into GenBank after 16S rRNA nucleic acid sequencing (DQ676505) [20]
Summary
Bioleaching technology is a newly developed hydrometallurgical technology [1]. is technique is cross-fused with the preparation of medicinal realgar and uses natural acidophilic microorganisms to perform bioleaching [2]. Compared to traditional preparatory methods, the bioleaching of realgar can significantly increase its solubility and bioavailability. This method is highly efficient, ecofriendly, and of low cost [5]. In the bioleaching of realgar, an important aspect is monitoring the changes in the concentration of arsenic during the process [2]. The molybdenum blue spectrophotometric method is utilized to measure the arsenic content. The experimental setup does not allow high-throughput processing in the case of high amounts. Another commonly implemented method utilizes instrumental analysis by inductively coupled plasma atomic emission spectroscopy (ICP-AES). ICP-AES is safer and more sensitive, but the instrumentation is relatively expensive, and, more importantly, it is difficult to conduct in situ analysis
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