Abstract
This chapter discusses some practical considerations in the design of experiments using Li + , based on experience with pituitary gonadotrope cells in culture. These cells respond to the neuroendocrine peptide, gonadotropin-releasing hormone (GnRH), by the release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Experimental procedure is provided in the chapter. Determination of optimal [Li + ] and effects on inositol phosphate (IP) accumulation is also presented. Most investigators using whole-cell preparations have been treated with Li + at 5–10 m M. These concentrations are in severalfold excess of the K i (0.8 m M) reported by Hallcher and Sherman for the Li + inhibition of inositol-l-phosphate phosphatase (I-1-Pase) and are generally found to provide maximal IP signal stabilization. Li + concentration dependence of GnRH-stimulated IP accumulation is decsribed in the chapter. It is important that available physiological end points in an experimental system be evaluated with respect to the possible extraneous effects of Li + .
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