Abstract
A high-pressure liquid chromatography (HPLC) technique, previously developed for nitrite (NO−2) and nitrate (NO−3) measurements [3], was used to measure chlorite (ClO−2) production by Nitrobacter winogradskyi. The determination of ClO−2 by HPLC involves monitoring the column effluent with a UV detector at 214 or 254 nm. Although the absorbance of ClO−2 at 214 nm was about 5 times greater than at 254 nm, interference from other compounds in the culture filtrates of N. winogradskyi contributed to an unstable detector signal. The detection limit at 254 nm for ClO−2 in deionized water was about 1 μM. The measurement of ClO−2 in N. winogradskyi culture filtrates was done with detection at 254 nm. The maximum concentration of ClO−2 produced by anaerobically incubated cell suspensions of N. winogradskyi was about 80 μM.
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