Use of iQPR-H2O for bone regeneration and its potential in the improvement of osteoporosis

Chiming Lee,Wentien Hsiao,Gowlin Lam,Henyu Liu,Kwanghwa Chang,Mingfu Wang,Meileng Cheong,Chihhsiung Wu,Winping Deng,Chingyu Tsai

doi:10.1186/1471-2474-12-227

Abstract

BackgroundCurrent treatments for osteoporosis are associated with various side effects and do not prevent the age-related decrease in osteoblast number. The objective of this study was to evaluate the effects of iQPR-H2O on osteogenesis.MethodsMouse fibroblast NIH3T3 and pre-osteoblastic MC3T3-E1 cells were cultured in medium prepared with iQPR-H2O or unprocessed mineral water (control cells), and proliferation and differentiation were assessed by MTT and alkaline phosphatase assay, respectively. Mineral deposition by the cells was determined using Alizarin red S staining. A mouse model of osteoporosis, ovariectomized SAMP8 mice, was used to evaluate the effects of iQPR-H2O on osteogenesis in vivo. Mice were given either iQPR-H2O or unprocessed mineral water (control group) for four months after which bone mass density (BMD) measurements were made using a bone densitometer and hematoxylin and eosin staining of bone samples.ResultsNIH3T3 cells grown in medium prepared with iQPR-H2O exhibited significantly greater proliferation. NIH3T3 and MC3T3-E1 cells demonstrated a significant increase in alkaline phosphatase levels in the iQPR-H2O group. MC3T3-E1 cells showed mineralization at day 28. mRNA expression levels of both osteopontin and runt-related transcription factor 2 in MC3T3-E1 cells were higher in the iQPR-H2O group compared with the control group. After four months, significantly greater bone regeneration was evident in ovariectomized SAMP8 mice administered iQPR-H2O as compared with control group.ConclusionsiQPR-H2O may reduce the symptoms of osteoporosis by improving osteogenesis.

Highlights

Current treatments for osteoporosis are associated with various side effects and do not prevent the age-related decrease in osteoblast number
There were no significant differences in alkaline phosphatase (ALP) levels between the iQPR-H2O and control groups following short-term culture of NIH3T3 cells (Figure 2A)
After 14 and 28 days culture of NIH3T3 cells, significantly greater ALP levels were evident in the iQPR-H2O group compared with those in the control group (P < 0.05, Figure 2B)

Summary

Introduction

Current treatments for osteoporosis are associated with various side effects and do not prevent the age-related decrease in osteoblast number. Pharmaceutical treatments include bisphosphonates, hormones, parathyroid hormones, and Infinitesimal Quantum persistent reflection (QPRTM) element water(iQPR-H2O) is prepared by a steady device of matter energy, which is a patented technology developed by Mr Lam Gow Lin (New Technology Number M 273565; United States Patent Application 20060099120; http://www.freepatentsonline.com/y2006/ 0099120.html) based on the theory of controlling “time parameters, quantum movements, and specific spaces” [13]. Bonds of molecules are broken, thereby producing elements and small molecules after which they are rearranged, increasing the energy state of matter. This treatment can be applied to drinking water, cosmetics, wine, and medical treatments, thereby increasing their energy states

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