Abstract
Summary An elisa, using hot saline solution extracts (hss) of a less-mucoid variant (M—) strain of Brucella canis as antigen, was developed for detection of antibodies against B canis in dogs. The test was applied to 177 field serum samples previously tested by use of the 2-mercaptoethanol rapid slide agglutination test, 2-mercaptoethanol-tube agglutination test, and agar gel immunodiffusion containing hss and cytoplasmic antigens of B canis. Results indicated that this elisa seems to be highly specific (95.6%) and slightly less sensitive (93.8%). The hss obtained from B canis wild-type RM 6/66 also have been used, but in our study, it seemed to be unsuitable for use in elisa because of the high background values observed for sera with negative test results.
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