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Abstract
A new, simple immunochromatographic assay for rapid identification of Mycobacterium tuberculosis complex in liquid cultures has been developed. The principle of the assay is binding of the Mycobacterium tuberculosis complex specific antigen to the monoclonal antibody conjugated on the test strip. The aim of this study is evaluation of the performance of immunochromatographic assay in identification of Mycobacterium tuberculosis complex in primary positive liquid cultures of BacT/Alert automated system. A total of 159 primary positive liquid cultures were tested using the immunochromatographic assay (BD MGIT TBc ID) and the conventional subculture, followed by identification using biochemical tests.Of 159 positive liquid cultures, using the conventional method, Mycobacterium tuberculosis was identified in 119 (74.8%), nontuberculous mycobacteria were found in 4 (2.5%), 14 (8.8%) cultures were contaminated and 22 (13.8%) cultures were found to be negative. Using the immunochromatographic assay, Mycobacterium tuberculosis complex was detected in 118 (74.2%) liquid cultures, and 41 (25.8%) tests were negative. Sensitivity, specificity, positive and negative predictive values of the test were 98.3%; 97.5%; 99.15% i 95.12%, respectively. The value of kappa test was 0.950, and McNemar test was 1.00. The immunochromatographic assay is a simple and rapid test which represents a suitable alternative to the conventional subculture method for the primary identification of Mycobacterium tuberculosis complex in liquid cultures of BacT/Alert automated system.
Highlights
A rapid isolation and identification of mycobacteria in the clinical samples is essential for the effective treatment of the disease [ ]
The immunochromatographic assay is a simple and rapid test which represents a suitable alternative to the conventional subculture method for the primary identification of Mycobacterium tuberculosis complex in liquid cultures of BacT/Alert automated system
We evaluated the utility of the immunochromatographic test Tbc ID (Becton Dickinson, Sparks, MD) to identify Mycobacterium tuberculosis complex in the primary positive liquid cultures of the BacT/Alert system
Summary
A rapid isolation and identification of mycobacteria in the clinical samples is essential for the effective treatment of the disease [ ]. Introduction of the liquid culture automated systems has significantly shortened the cultivation time of mycobacteria [ ]. Liquid cultures positive for acid-fast bacilli (AFB) indicate the presence of mycobacteria, requiring discrimination between Mycobacterium tuberculosis complex and nontuberculous mycobacteria (NTM) [ , ]. Identification of Mycobacterium tuberculosis complex in liquid cultures is usually performed on subcultures of mycobacteria on solid media using biochemical tests [ , ]. The identification process which requires several weeks increases the turnaround time for reporting positive results, diminishing the value of liquid cultures [ ].
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