Abstract
Bacillus subtilis possessing a stereospecific menthyl esterase has been immobilized in a polyurethane foam and used for the hydrolysis of menthyl ester. The specific activity of cells immobilized in polyurethane foam decreased as the cell loading was increased. The aqueous content of the immobilized biocatalyst particles influenced the activity. The activity half-life of about 400 h observed for the immobilized biocatalyst is 66 × greater than that observed for free bacteria.
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