Abstract
IN view of the increasing interest in marine algae it seems apposite to record a method of treatment which has been found useful in preparing microtome sections of some Florideae with particularly firm thalli. Formalin-alcohol, although a good fixative for cytological purposes, tends to harden such thalli, making them difficult to cut by microtome. Moreover, in those species which have an abundance of mucilaginous substances between the cells, the sections, when floated on water, swell considerably, twist, and escape from their casing of paraffin-wax. These difficulties can be avoided by treatment with warm hydrochloric acid in the case of Chondrus crispus, Gigartina mamillosa, Gracilaria confervoides and Rhodymenia palmata.
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