Abstract
Use of Humanized Mouse Models to Investigate the Roles of Purinergic Signaling in Inflammation and Immunity
Highlights
Purinergic signaling comprises a network of extracellular nucleosides and nucleotides, cell surface adenosine (P1) and nucleotide (P2) receptors, and ecto-enzymes that together participate in cell-tocell communication (Giuliani et al, 2019)
To determine if the above paradigm is relevant to human graft-versus-host disease (GVHD), our groups have investigated the roles of purinergic signaling in Hu-peripheral blood mononuclear cell (PBMC)-NSG mice using small molecule antagonists/agonists of purinergic molecules and PBMCs from human donors encoding natural variants of the P2RX7 and ENTPD1 genes (Figure 1)
Due to the development of lethal GVHD in Hu-PBMC-NSG other studies of purinergic signaling in inflammatory and immune processes in these mice remain limited. Given these mice readily engraft human T cells, these mice present opportunities to study the role of purinergic molecules in human T cell activation, differentiation, migration and survival in vivo for up to 4 weeks prior to clinical GVHD development
Summary
Purinergic signaling comprises a network of extracellular nucleosides and nucleotides, cell surface adenosine (P1) and nucleotide (P2) receptors, and ecto-enzymes that together participate in cell-tocell communication (Giuliani et al, 2019). Use of Humanized Mouse Models to Investigate the Roles of Purinergic Signaling in Inflammation and Immunity.
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