Abstract
Tools to detect human norovirus infectivity have been lacking. Using human intestinal enteroid cultures inoculated with GII.Pe-GII.4 Sydney–infected fecal samples, we determined that a real-time reverse transcription PCR cycle threshold cutoff of 30 may indicate infectious norovirus. This finding could be used to help guide infection control.
Highlights
Use of Human Intestinal Enteroids to Detect Human Norovirus InfectivityMolecular nucleic acid tests, such as real-time reverse transcription PCR (rRTPCR), are widely used for laboratory diagnosis of norovirus RNA in clinical samples [2]
Tools to detect human norovirus infectivity have been lacking
The Study We examined the infectivity of human pandemic norovirus genogroup II genotype 4
Summary
Molecular nucleic acid tests, such as real-time reverse transcription PCR (rRTPCR), are widely used for laboratory diagnosis of norovirus RNA in clinical samples [2] These molecular assays are virus specific and their analytical sensitivity is high, but they cannot distinguish between infectious and noninfectious viruses. These dilutions mimicked a broad range of cycle threshold (Ct) values of a widely used diagnostic rRT-PCR to represent high to very low norovirus levels [5]. From 2014 through 2018, a total of 114 (6.5%) of 1,754 norovirus-positive fecal samples from patients admitted to the Prince of Wales Hospital, Sha Tin, Hong Kong, with
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