Abstract
Abstract The species identity of ‘Roy Davidson’, a popular commercial cultivar in the genus Pulmonaria, is uncertain. To determine to which species ‘Roy Davidson’ is related to, we used fifteen primer combinations for Fluorescent-Amplified Fragment Length Polymorphism (F-AFLP) analysis. UPGMA dendrograms generated from AFLP markers obtained from the primer combinations showed that ‘Roy Davidson’ shares more genetic information with P. longifolia than with P. saccharata. Several factors that can affect F-AFLP profiles (DNA isolation from leaf tissue, optimization of F-AFLP procedures, and the use of this method for genotyping pulmonarias) were also evaluated; the results are discussed in this paper.
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