Abstract
The principles of flow injection and dilution, in a mixing chamber, were applied in the development of a rapid method for the study of the interaction between micelles and fluorescent molecules. A single channel manifold, with a well stirred mixing chamber, was used in different studies on the interaction of fluorescent probes with surfactants. The appropriate dilution of concentrate surfactant solutions, in the presence of a fluorescent probe, with a carrier containing the same probe provided information about the effect of the surfactant concentration in the fluorimetric determination of 2-(4-amino-2-hydroxyphenyl)benzothiazole (PAS-S). The dilution of a concentrate solution of a fluorescent molecule in a flow stream containing a surfactant allowed the corresponding fluorimetric calibration graph to be established in few minutes, and from that the micellar enhancement of fluorescence (MEF), in different media, could be easily determined. These procedures were applied to the interaction between micelles of anionic, cationic and non-ionic surfactants and PAS-S. The results obtained were compared with those found in batch analysis.
Published Version
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