Use of Extreme Low Temperatures Against Adzuki Bean Weevil (Callosobruchus chinensis L., Coleoptera: Chrysomelidae) in Storage Management

Abstract

This study was conducted to determine the effect of low temperatures on the different biological stages of adzuki bean weevil (Callosobruchus chinensis L., Coleoptera: Chrysomelidae) in stored chickpea seeds. Different extreme low temperatures were tested for eggs, larvae, pupae and adults in all experiments with different exposure times. For larvae, pupae and adults, 100% death was obtained after 45 minutes at -16 °C, 30 minutes at -20 °C and 20 minutes at -26 °C. LT95 value was determined as 38.3, 39.0 and 36.8 minutes for larva, pupa and adult at -16 °C, respectively. LT95 value was determined as 27.4, 27.5 and 26.0 minutes for larva, pupa and adult at -20 °C, respectively. After 120 minutes at -20 °C, 100% mortality was achieved in the eggs and it was determined that the adults did not emerge from the eggs that were kept at -20 °C for 60 minutes. From these results, it is understood that extreme low temperatures are effective in all biological stages of adzuki bean weevil that cause damage in storage, and this method can be used safely against other pests that cause damage in storage.