Abstract
The general objective of present work has been the devel- opment and application of analytical methodologies based on the combination of UV spectra with chemometrics methods of experi- mental design for establishing the matrix of calibration and later analysis of the obtained results. The analytes selected for this study have been citric acid and ascorbic acid. The citric acid is a tri-car- boxylic organic acid that is present in most of the fruits, mainly in citrus like the lemon and the orange. Ascorbic acid (vitamin C) is an organic acid with antioxidant properties; their importance for the human immune system and for the prevention of various diseases is a matter of common knowledge.
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