Use of Exogenous Glutathione for Metabolism of Peroxidized Methyl Linoleate in Rat Small Intestine

Abstract

The potential effects of dietary glutathione on the metabolism of peroxidized lipid ingested in the diet were studied using everted sacs of rat small intestine and peroxidized methyl linoleate. Peroxidized methyl linoleate was added to the luminal side, and the appearance of thiobarbituric acid-reactive (TBA-reactive) material on the contraluminal side was measured. Incubation with N,N,bis(2-chloroethyl)-N-nitrosourea (BCNU) under conditions in which it inhibits the glutathione disulfide reductase/glutathione peroxidase system increased the appearance of TBA-reactive material, indicating that at least a portion of the TBA-reactive material passing through the epithelium is peroxide in nature. Adding glutathione (GSH) to the luminal side substantially decreased the appearance of TBA-reactive material on the contraluminal side, either without or with BCNU treatment. Inhibition of GSH transport and control experiments with GSH, peroxidized methyl linoleate and purified brush border membranes showed that this decrease was due primarily to uptake of luminal GSH and its use to support intracellular GSH-dependent reactions. Thus, the results indicate that exogenous GSH, which can exist in certain diets, can be taken up by the small intestine and used to protect against absorption of lipid peroxidation products.