Abstract
One critial point in the Laboratory Animal Science (CAL) is the non-invasive evaluation of Animal Welfare (BEA). The detection (predictive or diagnostic) of the presence of suffering in mice when used for scientific purposes is a gap. The manuscrip aim to create a technical-scientific form to preditive presence of suffering in mice lab, based on the principle of Refinement of the 3Rs and BEA monitoring. Our methodology was based in usual environmental enrichment the objects, the trapeze. From these observations, our study hypothesis emerged, which was, the creation of the #1 Trap prototype and the measurement through the daily counting of the number of trapeze use during 15 minutes. Experimental acute infection by Trypanosoma cruzi was experimental model in Swiss Webster mice. Our results described that, after division into four groups: [N] without infection and [Inf] mice infected and without treatment. The predictive statistical difference was observed on the 11th day post infection (dpi) when compared ([N]: 72±18.5 Events number/15min) and ([Inf]: 32±13.1 Events number/15min) (p ≤ 0.05). The 21st dpi was the most severe time point ([N]: 75±17.3 and [Inf]: 11±5.1 Events number/15min, respectively) (p ≤ 0.05). So, our results suggest the #1 Trap prototype has predictive and monitoring capacity for experimental disease course. It makes it possible to prevent suffering and preservation of mice welfare during acute T. cruzi experimental model.
Highlights
Despite detailed studies on the genus Mus, or Mus musculus species, their genealogy, phylogeny, and historical aspects of the lineage origin currently used in the laboratory, including classical inbred lineages, the lack of documentation and scientific reports become imprecise in explaining exactly which species
We studied to structure a protocol that could be applied in experimental models and capable of predicting the onset of animal discomfort and suffering and/or diagnosing the animals that need to be removed from the trial and promoted end point (Figure 5)
We propose to prototype #1 Trap in a well-described experimental model, the Swiss Webster infected by the Y strain of T. cruzi (Figure 6)
Summary
Despite detailed studies on the genus Mus, or Mus musculus species, their genealogy, phylogeny, and historical aspects of the lineage origin currently used in the laboratory, including classical inbred lineages, the lack of documentation and scientific reports become imprecise in explaining exactly which species (or subspecies). Its origin is related to 200 mice from the Pasteur Institute (France) by Amédée Borrel provided by an unknown person. These animals were provided to André de Coulon of the Anticancereux Romand Center in Lausanne (Switzerland) [2]. An oncologist researcher of the Rockefeller Institute (New York) imported two male and seven female mice from André de Coulon. In the laboratory all animals derived from these mice were known as the “swiss” a nickname used by Lynch in her laboratory. Lynch collaborated and gave some of his new mouse, of the “swiss” to other researchers, both internally and to other institutions [3]
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