Use of Enterovirus Typing Pools for Immuno-Electron Microscopy

Abstract

The most common viruses found in foods are the enteroviruses, which because of the large number of serotypes and lack of group antigens, require the use of individual antisera to identify each virus. The standardized Lim Benyesh-Melnick enterovirus typing pools, distributed by the NIH, reduce the number of tests required to identify most of the enteroviruses of human origin. The 8 pools are capable of identifying 37 enteroviruses. We have adapted these typing pools for immuno-electron microscopy (IEM), to replace more timeconsuming serological tests.