Use of ELISA technique for detection of streptomycin residues in milk

Abstract

This study involved examination of 45 milk samples were distributed as: 15 imported milk powder, 10 imported liquid milk, 10 local cow's milk, 10 local sheep's milk. These samples were collected from September 2010 to April 2011 at different markets in Mosul city (Iraq) in order to detect level of streptomycin residue using Enzyme Linked ImmunoSorbent Assay (ELISA) technique. The results showed that the percentage of positive samples of streptomycin residue in milk was 53.3%, and as follow 15.6% in the local sheep's milk samples, 13.3% in the local cow's milk samples, 11.1% in the imported liquid milk samples and 13.3% in the imported milk powder samples with mean value of 22.8, 20.1, 12.6 and 19.9 µg/kg milk, respectively. From these results, it is clear that all samples of milk had residual streptomycin level within the permissible limits and it is regarded as safe for human consumption.