Abstract
An ELISA was developed for Phaeosphaeria typharum (Desm.) Holm. The assay could detect 0.1 μg dry fungal organic matter·ml −1. Cross-reactivity occurred with some fungal species common to the salt-marsh but not with nonfungal materials. Mycelial age had only a moderate effect on the ELISA response. Negative interference in the ELISA of standing-dead brown leaves caused a 30% decrease in sensitivity. In a 9-month field experiment, results from the ELISA indicated that, at peak development. P. typharum biomass could account for >10% of the C in standing-dead leaves of smooth cordgrass Spartina alterniflora Loisel.
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