Abstract
Neosporosis is a disease caused by the protozoon Neospora caninum that leads to significant economic losses in many countries. In the present study, we report on use of the recombinant protein NcSRS2 of N. caninum expressed in Pichia pastoris in an indirect immunoenzymatic assay (ELISA) for diagnosing neosporosis infection in sheep and dogs. We observed that the ELISA test yielded specificity of 94.5% and sensitivity of 100% for sheep and specificity of 93.3% and sensitivity of 100% for dogs. We observed that the sensitivity was higher than shown by the indirect fluorescent antibody test, and this was confirmed by means of Western blot. The results from this study suggest that the recombinant protein expressed in P. pastoris is a suitable antigen for use in immunodiagnosis to detect N. caninum in two important species exposed to this parasitosis.
Highlights
Dogs are essential to the life cycle of Neospora caninum (DUBEY et al, 2007)
We report on use of the recombinant protein NcSRS2 of N. caninum expressed in Pichia pastoris in an indirect immunoenzymatic assay (ELISA) for diagnosing neosporosis infection in sheep and dogs
The results from this study suggest that the recombinant protein expressed in P. pastoris is a suitable antigen for use in immunodiagnosis to detect N. caninum in two important species exposed to this parasitosis
Summary
Dogs are essential to the life cycle of Neospora caninum (DUBEY et al, 2007). They are both the definitive and the intermediate host, and are epidemiologically important for horizontal transmission to other food animals (GONDIM et al, 1999). Several studies have been conducted to identify and characterize the molecular antigenic components of N. caninum, in order to improve the performance of serological diagnostic tests and to reveal the mechanisms of its host interactions (HEMPHILL et al, 1999). Information on the antigenicity of Apicomplexa-specific antigens, including in relation to Neospora, is needed in order to avoid cross-reactions with parasites from the same phylum (DUBEY et al, 2003). Serological tests that use this category of www.cbpv.com.br/rbpv v. 24, n. 2, abr.-jun. 2015
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Schedule a callMore From: Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria
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