Use of electroencephalogram (EEG) to optimize stunning efficiency and animal welfare in commercial catfish production

Abstract

The welfare of farmed fish has gained increasing attention during recent decades, and as technological advances have facilitated measurements of brain activity in fish, the slaughter process has been highlighted as an area for assessment and potential improvement. Here, we used electroencephalograms (EEG) to assess brain activity in channel catfish (Ictalurus punctatus), and used commercial slaughter practices to guide optimization of stunning conditions in a laboratory setting. Following in-air electro-stunning at processing plants, individual fish responses to the shock varied based on EEG and corresponding ventilation measurements prior to physical euthanasia. Results from laboratory experiments showed stunning efficacy is dependent on shock duration and the location where electrodes contact fish. Electrodes contacting the head for 1 s using 50 Hz 132 AC VRMS, with a current >380 mARMS, caused immediate loss of consciousness lasting 10–40 s. When the exposure period was prolonged to 6 s, recovery time was significantly longer, ranging from 45 to 240 s (mean 125 s). If the electrodes contacted the body instead of the head, shock delivered for 6 s resulted in a shorter recovery time of 0–100 s (mean 48 s). These findings highlight that shock duration and electrode position are important when stunning channel catfish and presumably other fishes, and indicate the time from stunning to killing should be kept as short as possible.