Use of Dual Detection in the Gas Chromatographic Analysis of Oleaginous Biomass Feeds and Biofuel Products To Enable Accurate Simulated Distillation and Lipid Profiling

Abstract

This contribution describes the development of a chromatographic method capable of simultaneously identifying and quantifying the constituents found in oleaginous biomass—including algae oil—and in biofuel samples through a single cost-effective analysis. Major constituents of the aforementioned analytes include oxygen- and/or nitrogen-containing compounds along with fuel-like hydrocarbons. A novel simulated distillation gas chromatographic (SimDist) technique including dual detection capabilities—specifically flame ionization (FID) and mass spectrometry (MS)—produced identical chromatograms with perfectly aligned signals. FID chromatograms afforded excellent quantitative data while the corresponding MS spectra enabled accurate and thorough compound identification. Simulated distillation data displayed a remarkably linear relationship between retention time and the boiling point of heteroatom-containing compounds in addition to n-alkanes. Indeed, although standard SimDist data were calibrated using the bo...