Use of dietary tea seeds to manipulate the rumen ciliate protozoa population in sheep

Abstract

Eighteen rumen cannulated sheep in three groups of six were supplemented with tea seeds at 0 (control), 3 or 6% of the dietary dry matter in a 22-d experiment. Rumen fluid samples were collected on 3 d before and on various days during the experiment. The concentration of protozoa in rumen fluid was not affected by the tea seed supplements (P > 0.05), but the numerical concentration of the Entodinium genera declined by 12% (P < 0.05) and that of cellulolytic protozoa increased by 90% (P < 0.05) due to the 6% tea seed supplement. The associated percentage in the rumen ciliate protozoa population of the cellulolytic protozoa increased (P < 0.05) and that of the Entodinium genera decreased (P < 0.05) due to the 6% supplement. There were no effects (P > 0.05) of the 3% tea seed supplement on the protozoa population parameters. Since ammonia and volatile fatty acid concentrations were not affected (P > 0.05) it was apparent that the tea seed supplements did not influence rumen fermentation. The positive effect of the 6% tea seed supplement on the ciliate protozoa population obtained in the present study was relatively modest. Because the seeds contain astringent properties, higher than 6% dietary supplements of tea seeds can be harmful to animals. It was therefore concluded that tea seeds are of no apparent value as an antiprotozoal dietary component in ruminant production. Key words: Tea seeds, ciliate protozoa, rumen fluid, sheep