Use of Detached Leaves to Evaluate Tobacco Haploids and Doubled Haploids for Resistance to Tobacco Mosaic Virus,Meloidogyne incognita,andPseudomonas syringaepv.tabaci

Abstract

Rufty, R. C., Wernsman, E. A., and Gooding, G. V., Jr. 1987. Use of detached leaves to evaluate tobacco haploids and doubled haploids for resistance to tobacco mosaic virus, Meloidogyne incognita, and Pseudomonas syringae pv. tabaci. Phytopathology 77:60-62. Development of disease-resistant cultivars in self-pollinated crops like virus Y. Detached leaves were maintained by immersing their petioles in tobacco (Nicotiana tabacum) can be greatly accelerated by evaluating water until symptoms appeared. Symptoms in detached leaves were similar populations of haploid plants derived from F1 hybrids resistant to various to those in intact plants, and disease reactions corresponded with wholediseases. A limitation of haploid breeding is the need to assess reactions to plant determinations. The technique can also be used with doubled haploid multiple pathogens on single plants. To avoid confounding systemic or or diploid populations segregating for disease resistance. The original lethal effects from inoculations with multiple pathogens, detached leaves intact plants with identified resistance may be evaluated for other traits, were inoculated separately with tobacco mosaic virus and Pseudomonas and susceptible genotypes may be discarded. Field evaluations for syringae pv. tabaci. Resistance to Meloidogyne incognita was identified by agronomic characteristics can then be performed on a population fixed for the associated reactions of detached leaves to the MsN R strain of potato disease resistance genes.