Abstract
One-step reverse transcription-polymerase chain reaction (one-step RT-PCR) was successfully adapted to detect Bean yellow mosaic virus in the crude sap of infected dwarf gentian plants (Gentiana scabra). The virus was detected directly from crude sap at dilutions up to 106-fold (W/V), which is 100 times more sensitive than indirect enzyme-linked immunosorbent assay or bioassay after mechanical inoculation, and equally as sensitive as immunocapture RT-PCR. This one-step RT-PCR method using crude sap was also useful for detecting 16 virus species from ten genera with isometric, filamentous, and rod-shaped particles from 32 plant species in 15 families.
Published Version
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