Use of Conventional Bioanalytical Devices to Automate DBS Extractions in Liquid-Handling Dispensing Tips

Abstract

Conventional liquid-handling devices were employed, along with an improved punching device, to semi-automate dried blood spot (DBS) extraction of alprazolam, α-hydroxyalprazolam and midazolam from human whole blood. Liquid-handling devices were used to add internal standard to the DBS cards and to extract the analytes from the DBS, in order to be analyzed by HPLC-MS/MS. The technique was shown to be accurate (±12.0%) and precise (10.3%) across the dynamic range of the assay. The semi-automated extraction reduced sample preparation time by more than 50% when compared with more conventional DBS manual extraction methods.