Use of Comet and Micronucleus Assays to Measure Genotoxicity in Meadow Voles (Microtus pennsylvanicus) Living in Golf Course Ecosystems Exposed to Pesticides

Abstract

The purpose of this study was to conduct a biomonitoring study to measure the effects of pesticide exposure in meadow voles (Microtus pennsylvanicus) living in golf courses of the Ottawa/Gatineau region of Canada. In this article we present the results from the comet and micronucleus assay. Voles were captured in 2001 and 2002 at five golf courses and two reference sites. Blood was collected from sedated voles. Three animals from each course were euthanized to determine body burdens of historically used organochlorine (OC) and metal-based pesticides. Exposure to in-use pesticides was determined from detailed golf course pesticide use records. Comet tail length and tail moment were not related to body burdens of OC pesticides and metals historically used on these golf courses. In generally, tail length and moment significantly decreased in relation to days since last application of a pesticide, and to days since the last application of a specific fungicide (Daconil) containing a potentially genotoxic active ingredient (chlorothalonil). The slopes of these curves in 2002 were not significantly different than the half-life decay curve of chlorothalonil on vegetation. Both comet assay parameters appeared to increase in a dose-dependent manner with the amount of the last application Daconil. The number of micronucleated polychromatic erythrocytes was not related to any pesticide application parameter.