Abstract
We propose a method to utilize colloidal quantum dots (QDs) as a swept light source for hyperspectral microscopy. The use of QD allows for uniform multicolor emission which covers visible-NIR wavelengths. We used 8 colors of CdSe/ZnS and CdTe/ZnS colloidal quantum dots with the peak emission wavelengths from 520 nm to 800 nm. The QDs are packed in a compact enclosure, composing a low-cost, solid-state swept light source that can be easily used in most microscopes. Multicolor emission from the QDs is simply controlled by digitally switching excitation UVLEDs, eliminating the use of mechanically-driven gratings or filters. We used gold nanoparticles as optical markers for hyperspectral microscopy. Due to the effect of localized surface plasmon resonance, gold nanoparticles demonstrate size and shape-dependent absorption spectra. Employed in a standard microscope, the QD light source enabled multispectral absorption imaging of macrophage cells labeled with gold nanorods and nanospheres.
Highlights
Hyperspectral imaging is a technique where spectral information is recorded over many wavelengths for each pixel of a high-resolution microscopic image [1,2]
We propose a method to utilize colloidal quantum dots (QDs) as a swept light source for hyperspectral microscopy
The use of QD allows for uniform multicolor emission which covers visible-NIR wavelengths
Summary
Hyperspectral imaging is a technique where spectral information is recorded over many wavelengths for each pixel of a high-resolution microscopic image [1,2]. A swept light source is a key element in hyperspectral microscopy It usually consists of a wide band light source coupled with mechanically motorized color wheels or gratings coupled with mechanically scanned stages [1,2], which make the setup complicated and expensive (see Fig. 1(a)). The use of QDs allows for easy construction of a compact swept light source that uniformly covers visible-NIR wavelength range. Another unique advantage of the QD based swept light source is that it does not contain any moving parts.
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