Use of Chitosan to Prevent Aeromonas hydrophila Infection on Catfish Clarias sp.

Abstract

<p>Immunostimulation effect of chitosan against motile aeromonas septicemia caused by <em>Aeromonas hydrophila</em> were examined in catfish (<em>Clarias </em>sp<em>.</em>). Experimental fish were injected with 2, 4 and 6 μg/g fish of chitosan. All fish were subsequently challenged by 10<sup>5</sup> CFU/ml of live <em>A. hydrophila</em> by injection method. Negative control injected with PBS and positive control injected with only <em>A. hydrophila</em> were included in the experiment. Results showed that total count of eritrocyte, leucocyte, level of hematocrite, haemoglobin and phagocytic index higher at fish injected with chitosan previously compared with control as well as lymphocyte, neutrophile, monocyte, and trombocyte. Either survival rate or growth of fish injected with chitosan were found to increase in accordance with dose of chitosan.</p> <p>Kata Kunci : chitosan,<em> Aeromonas hydrophila</em>, immunostimulant, <em>Clarias </em>sp.</p> <p> </p> <p>ABSTRAK</p> <p>Efek imunostimulasi dari kitosan melawan <em>Motile Aeromonad Septicemia </em>yang disebabkan oleh <em>A. hydrophila</em> dilihat pada ikan lele (<em>Clarias</em> sp.). Ikan uji disuntik dengan larutan kitosan dengan dosis 2, 4 and 6 μg/g, yang selanjutnya diuji tantang dengan bakteri <em>A. hydrophila</em> 10<sup>5</sup> CFU/ml melalui penyuntikan intramuskular. Kontrol negative disuntik dengan PBS dan control positif disuntik hanya dengan bakteri <em>A. hydrophila</em> disertakan dalam penelitian ini. Hasil menunjukkan bahwa jumlah eritrosit, lekosit, level hematokrit, hemoglobin dan indeks fagositik lebih tinggi pada ikan-ikan yang diberi kitosan sebelumnya dibandingkan dengan tanpa pemberian kitosan sebelumnya. Begitu pula dengan kadar limfosit, netrofil, monosit dan trombosit. Sintasan dan pertumbuhan ikan yang diberi kitosan meningkat sejalan dengan dosis kitosan yang diberikan.</p> <p>Kata kunci: kitosan,<em> </em><em>Aeromonas hydrophila</em>, immunostimulan, <em>Clarias </em>sp.</p>