Abstract
An antagonistic bacillus S2BC-2 isolated from apple rhizosphere soil was identified to be highly chitinolytic on chitinase detection agar. Standard bacteriological tests and sequencing of 16S rRNA, and gyrA and rpoB genes, indicated a taxonomic affiliation of the strain to Bacillus atrophaeus. The strain was studied for its ability to grow and produce chitinase on different substrates. Bacterial cells grown on chitin-containing media showed enhanced growth and chitinase production with increased anti-fungal activity against vascular wilt pathogens. Extracellular proteins of cell-free extracts of media amended with chitin and fungal cell wall contained 4–10 novel polypeptides. In polyhouse (bamboo structures that provide protective shade made of polyvinyl sheet) studies, a chitin-supplemented talc-based formulation of the S2BC-2 challenge inoculated with Fusarium oxysporum f. sp. zingiberi recorded low percent disease indices of 84.9 % and 79.2 % for yellows and rhizome rot, respectively, over the non-bacterised pathogen control. The low disease incidences correlated with 113.3 % maximum rhizome production and 2-fold higher chitinase induction over the pathogen control. In native gel activity assays, upon challenge-inoculation, S2BC-2 expressed more chitinase isoforms than the pathogen control. The results suggest that chitinolytic B. atrophaeus can be used in the biocontrol of rhizome rot of ginger.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
