Abstract
Summary Reversed-phase high-performance thin-layer chromatography (HPTLC) has been carried out on LiChrosorb RP-2, RP-8 and RP-18, and Whatman KC18 precoated thin-layer plates. With methanol or acetonitrile containing 0–30 % water as the mobile phase, the KC18 and RP-type HPTLC plates perform equally well, the behaviour of the KC18 plates being very similar to that of the RP-8 plates. With mobile phases containing more than ca. 30 % water, RP-coated HPTLC plates cannot be used conveniently due to non-wettability of the stationary phase. KC18 plates can be used irrespective of the proportion of water, provided 3 % NaCl is added to the mobile-phase solvent mixture. Various separations are shown, e.g., of phthalate esters, chloroanilines, aromatic hydroxy compounds and polynuclear aromatic hydrocarbons.
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