Abstract
A polycationic derivative of ferritin for labeling of negative charges on cell surfaces was prepared by coupling horse spleen ferritin with N, N dimethyl-1,3 propanediamine (DMPA) via carbodiimide activation of the protein carboxyl groups. Various derivatives of cationized ferritin prepared in the pH range between 5 and 7 were characterized and tested for their labeling capacity. The DMPA-ferritin prepared at pH 6.5 was chosen as the optimal preparation for the present study. Labeling with cationized ferritin is performed at physiological conditions with or without prefixation with glutaraldehyde. It is effective on a variety of cells, and its geometry permits relatively easy counting on the membrane surface. The difference in surface charge labeling between young, old, and receptor-destroying enzyme (RDE)-treated rabbit red cells is reflected in the number of cationized ferritin particles attached per unit area of membrane in tangentially sectioned red cells. This is in agreement with the values obtained by electrophoresis.
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