Abstract
To evaluate the ability of macroporous tricalcium phosphate cement (CPC) scaffolds to enable the adhesion, proliferation, and differentiation of mesenchymal stem cells derived from human bone marrow. Cells from the iliac crest of an adult human donor were processed and cultured on macroporous CPC discs. Paraffin spheres sized between 100 and 250µm were used as porogens. Cells were cultured for 5, 10, and 15 days. Next, we assessed cells' behavior and morphology on the biomaterial by scanning electron microscopy. The expression levels of the BGLA and SSP1 genes and the alkaline phosphatase (ALP) activity were quantified by the quantitative real-time polymerase chain reaction technique (QT-PCR) using the fluorophore SYBR GREEN(®). QT-PCR detected the expression of the BGLA and SSP1 genes and the ALP activity in the periods of 10 and 15 days of culture. Thus, we found out that there was cell proliferation and differentiation in osteogenic cells. Macroporous CPC, with pore sized between 100 and 250µm and developed using paraffin spheres, enables adhesion, proliferation, and differentiation of mesenchymal stem cells in osteogenic cells and can be used as a scaffold for bone tissue engineering.
Highlights
The strategies commonly used to treat bone defects in the maxillofacial region include autogenous bone grafts, allografts, metals and ceramics
Much attention has been paid to the use of calcium phosphate cement (CPC) as a bone substitute
The main objective of this study was to assess the viability of using a CPC made of Brazilian raw material, with porosity created by paraffin spheres, as a scaffold for bone tissue engineering by analyzing the behavior of mesenchymal stem cells cultivated in osteogenic induction medium on this biomaterial
Summary
The strategies commonly used to treat bone defects in the maxillofacial region include autogenous bone grafts, allografts, metals and ceramics All these alternatives have specific limitations such as restricted amount of tissue obtained with autogenous grafts, difficulty of preparing grafts with the desired shape, and risk of transferring pathogens when using synthetic implants and allograft[1]. Much attention has been paid to the use of calcium phosphate cement (CPC) as a bone substitute. Such biomaterial has a composition and structure very similar to the mineral portion of the bone tissue and has been considered appropriate to develop scaffolds for bone tissue engineering[4]. In addition to these characteristics, CPCs are handled and molded
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
