Abstract

AbstractThe objective of this research was to use biosurfactant-producing bacteria in bioremediation of oil-contaminated soil. Two approaches were studied. Biostimulation with the addition of crude biosurfactants extracted from Rhodococcus fascians, strain already isolated from hydrocarbon contaminated soils, and bioaugmentation with the addition of bacterial cells suspensions of the same strain. The bacterial quantification results revealed significant microbial abundance in both treatments. The results of soil hydrocarbon analysis revealed an efficiency of hydrocarbons elimination in both treatments (>30%). Metals analysis showed significant results of metals removal using both treatments (>75% for chromium). The ecotoxicity test showed an important decrease of oil-contaminated soil toxicity using the two treatments (>55%). Both approaches used showed an efficient elimination of hydrocarbons and metals and represented an environmentally friendly technology.KeywordsBiosurfactantsBioremediationHydrocarbonsMetalsPetroleumBacterial cells

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